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AAV »ý»ê·®À» ³ôÀÌ´Â transfection enhancer

RevIT¢â AAV Enhancer

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 8000
RevIT¢â AAV Enhancer
°ü·ÃÇмú ±¸¸ÅÇϱâ
1.5 ml (1L cell culture)
°¡°Ý¹®ÀÇ   revit-aav-enhancer.pdf
Mirus
MIR 8006
RevIT¢â AAV Enhancer
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 x 1.5ml (10L cell culture)
°¡°Ý¹®ÀÇ  
Mirus
MIR 8007
VirusGEN¢ç AAV Transfection Kit with RevIT¢â AAV Enhancer
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 kit (1L cell culture)
°¡°Ý¹®ÀÇ   virusgen-aav-transfection-kit-with-revit-aav-enhancer-protocol.pdf
Mirus
MIR 8008
VirusGEN¢ç AAV Transfection Kit with RevIT¢â AAV Enhancer
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 kit (10L cell culture)
°¡°Ý¹®ÀÇ  

*º» Á¦Ç°Àº VirusGEN¢ç AAV Transfection Kit (Code MIR 6750)ÀÇ ¾÷±×·¹À̵å Á¦Ç°ÀÔ´Ï´Ù .
Á¦Ç° ¼³¸í
RevIT¢â AAV Enhancer´Â ±âÁ¸¿¡ »ç¿ëÇÏ´ø transfection ½Ã¾à¿¡ ÷°¡ÇÏ¿© AAVÀÇ »ý»ê·®À» ³ô¿©ÁÖ´Â ½Ã¾àÀ¸·Î, serotype, ¼¼Æ÷ ¹è¾ç ¹èÁö ¹× transfection ½Ã¾àÀÇ Á¾·ù¿Í °ü°è¾øÀÌ 2~4¹è ³ôÀº ¿ª°¡·Î AAV¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù. AAV Á¦Á¶ °øÁ¤ ½Ã transfection ½Ã¾à°ú ÇÔ²² RevIT¢â AAV Enhancer¸¦ Ãß°¡ »ç¿ëÇÔÀ¸·Î¼­ °íÇ°ÁúÀÇ ¿ª°¡¸¦ À¯ÁöÇÏ¸ç ´õ ÀûÀº ¾çÀÇ plasmid DNA¸¦ »ç¿ëÇÒ ¼ö ÀÖ°Ô µÇ¾î ÈÄ¼Ó Á¦Á¶ ºñ¿ëÀ» ´õ¿í Àý°¨ÇÏ´Â µî AAV Á¦Á¶ °øÁ¤À» °³¼±ÇÒ ¼ö ÀÖ´Ù.
Á¦Ç° ±¸¼º

Code

RevIT¢â
AAV Enhancer

TransIT-VirusGEN¢ç
Transfection Reagent

MIR 8000

1.5 §¢

-

MIR 8006

10 x 1.5 §¢

-

MIR 8007

1.5 §¢

2 x 1.5 §¢

MIR 8008

10 x 1.5 §¢

1 x 30 §¢



±×¸² 1. RevIT¢â AAV titer enhancerÀÇ AAV »ý»ê Áõ´ë È¿°ú
Polymer based transfection ½Ã¾à¿¡ RevIT¢â AAV titer enhancer¸¦ º´Çà »ç¿ëÇÏ¸é ´Ù¾çÇÑ serotypeÀÇ AAV¸¦ ´õ¿í ³ôÀº ¿ª°¡·Î »ý»êÇÒ ¼ö ÀÖ´Ù.


±×¸² 2. ´Ù¾çÇÑ ¹èÁö ¹× cell line¿¡ Àû¿ë °¡´ÉÇÑ RevIT¢â AAV titer enhancer
´Ù¾çÇÑ Å¸»ç ¹èÁö ¹× virus »ý»ê¿ë packaging cell line¿¡¼­µµ RevIT¢â AAV titer enhancer¸¦ ÷°¡ÇÏ´Â °æ¿ì ´õ ³ôÀº ¼öÀ²·Î AAV¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù (TransIT-VirusGEN¢ç Transfection ½Ã¾à°ú ÇÔ²² »ç¿ë).


±×¸² 3. TransIT-VirusGEN¢ç Transfection Reagent¿Í RevIT¢â AAV titer enhancer Á¶ÇÕÀÇ AAV »ý»ê Áõ´ë È¿°ú
RevIT¢â AAV titer enhancer¸¦ polymer based transfection ½Ã¾à ¶Ç´Â MirusÀÇ TransIT-VirusGEN¢ç Transfection Reagent¿Í ÇÔ²² »ç¿ëÇÏ¿© AAV¸¦ »ý»êÇÑ °á°ú, TransIT-VirusGEN¢ç Transfection Reagent ¿Í º´Çà »ç¿ëÇÑ °æ¿ì¿¡ °¡Àå ³ôÀº ¿ª°¡ÀÇ AAV¸¦ »ý»êÇÒ ¼ö ÀÖ¾ú´Ù.


±×¸² 4. ±âÁ¸ AAV enhancer º¸´Ù ³ô¾ÆÁø ¼º´É
±âº» ½Ã¾àÀÎ TransIT-VirusGEN¢ç Transfection Reagent¿Í ±âÁ¸ Á¦Ç°ÀÎ VirusGEN¢ç AAV Transfection Kit (Enhancer 1 Æ÷ÇÔ) ¸¦ ºñ±³ÇÑ °á°ú, ½ÅÁ¦Ç°ÀÎ VirusGEN¢ç AAV Transfection Kit with RevIT¢â AAV Enhancer ¸¦ ÀÌ¿ëÇÏ¿´À» ¶§ ´õ ³ôÀº ¼öÀ²·Î AAV¸¦ »ý»êÇÒ ¼ö ÀÖ¾ú´Ù.
º¸Á¸
TransIT-VirusGEN¢ç Transfection Reagent: -10~-30¡É º¸Á¸
RevIT¢â AAV titer enhancer* : -10~-30¡É º¸Á¸
(*5ȸ ÀÌ»óÀÇ µ¿°áÀ¶ÇØ ±ÝÁö)
°ü·ÃÁ¦Ç°
- AAV/Lentivirus »ý»ê Àü¿ë: TransIT-VirusGEN¢ç Transfection Reagent
- AAV titrationÀÇ gold standard: AAVpro¢ç Titration Kit (for Real Time PCR)
- ´Ù¾çÇÑ SerotypeÀÇ vector system: AAVpro¢ç Helper Free System (AAV1, 2, 5, 6)
- CRISPR/Cas9 Vector System: AAVpro¢ç CRISPR/Cas9 Helper Free System (AAV2)
- AAV extraction: AAVpro¢ç Extraction Solution
    AAVpro¢ç Freeze-Thaw Extraction Buffer (All Serotypes)
- ¹è¾ç¹èÁö¿Í cell¿¡¼­ ¸ðµÎ Á¤Á¦: AAVpro¢ç Cell & Sup. Purification Kit Maxi (All Serotypes)
- Packaging (293T Cell Line): AAVpro¢ç 293T Cell Line

Keyword :

È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
>
°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ..
TransIT-VirusGEN¢ç Transfection Reagent
>
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
TransIT¢ç-Lenti Transfection Reagent
>
AAV »ý»ê·®À» ³ôÀÌ´Â transfection enhancer
RevIT¢â AAV Enhancer
>
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
>
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit